Mad1对膀胱肿瘤作用的体内实验研究

Experimental study on the effects of Mad1 to bladder tumor in vivo

目的:在原位建立膀胱肿瘤的大鼠模型,观察转染Mad1表达质粒后对膀胱肿瘤增殖的抑制作用。方法:MNU对SD大鼠进行膀胱灌注诱发膀胱肿瘤,荷瘤动物随机分为3组:Mad1转染组(A组),空载体转染组(B组)和生理盐水转染组(C组)。通过大鼠重量(RBW)、膀胱绝对重量(BAW)、膀胱相对重量(BRW)、Mad1和TERT mRNA表达水平等指标,用流式细胞仪等检测手段,比较转染前后Mad1和TERT的表达差异、观察Mad1对膀胱肿瘤增殖的抑制作用以及探讨相应的机理。结果:成功在原位建立膀胱肿瘤的大鼠模型。A组与B、C两组相比RBW无显著性差异(P>0.05),但BAW和BRW分别有非常显著差异性(P<0.01)及显著差异性(P<0.05);Mad1mRNA相对表达量明显增加;TERT mRNA相对表达量明显降低;流式细胞仪显示G0/G1期细胞显著增加(P<0.01),S期细胞显著减少(P<0.01).结论:高表达的Mad1能抑制膀胱肿瘤的增殖。

Objective：To construct the orthotopic model of bladder tumor in rats and to ohserve the inhibition effect on bladder tumor after transfected with expression plasmid pcDNA3.1 （＋）/Madl. Methods：Bladder tumors were induced in SD rats by intravascular instillation of MNU. The rats bearing tumor were randomly divided into 3 groups：transfected with pcDNA3.1 （＋） /Madl （group A）,transfected with empty vector （group B） and transfected with saline （group C）. Tumor-hearing rats were transfected using Lipofectamine. Rat body weight （RBW）,Bladder absolute weight （BAW）,bladder relative weight （BRW） and expression levels of mRNA of Madl and TERT were assayed and flow cytometer analysis were used to ohserve the inhibition effect of Madl to bladder tumor. Results：Compared with group B and group C,in group A,though there was no diference in RBW（P〉0.05）,BAW and BRW were significantly decreased （P〈0.01 and P〈0.05,respectively）. Madl mRNA expression levels were markedly improved,while TERT mRNA expression levels were markedly decreased. Flow cytometry showed an increase of G0/G1-phase cells and a decrease of S-phase cells after transfected with Madl. Conclusions：Over expression of Madl can inhibit the proliferation of bladder tumor.