鹅细小病毒QY分离株NS2基因的克隆及序列分析

Cloning and sequencing of NS2 genes of goose parvovirus QY strain

参照GenBank中收录的鹅细小病毒(GPV)B株基因序列设计并合成了扩增广东清远分离株(QY株)NS2基因的一对引物,利用PCR技术扩增出来长约1.5kb的目的片断,将其克隆到PMD18-T载体上,筛选重组质粒并进行了序列测定及分析,测序结果表明,GPV分离株NS2基因由1356个核苷酸组成,编码451个氨基酸。经与B株、DN株、FS株进行同源性比较,核苷酸的同源性分别为99.1%、99%和99%;推导的氨基酸同源性分别为98.7%、98.7%和987%。

According to the published genome primer was designed to amplify NS2 gene from goose sequence of goose parvovirus B strain ,oen pair of parvovirus Qingyuan （QY） strain （from Guangdong Province）by PCR. 1.Skb segment was amplified. The PCR products were cloned into pM D 18-T vector.I- dentified and sequenced ,Nucleotide sequence of NS2 genes of the QY strain was 1356bp in length ,encoding 451 amino acids .In comparison with the B Strain , DS strain and FS strain ,the nucleotide homology were 99.1%,99% and 99% respectively ,and the nucleotide homology of NS2 gene were 98.7%,98.7% and 98.7% respectively.