神经干细胞定向分化为少突胶质细胞的实验研究

Induction of neural stem cells into oligodendrocytes

目的:通过体外诱导使神经干细胞定向分化为少突胶质细胞,为从神经干细胞来源得到少突胶质细胞提供实验基础。方法:体外分离培养新生大鼠神经干细胞,加入甲状腺素(T3)和(或)胰岛素诱导分化,免疫细胞化学方法检测诱导末期NSC分化中GalC,GFAP的表达,通过比较增殖情况、分化率、GalC阳性细胞突起长度分析不同诱导因素组合对NSC定向诱导为少突胶质细胞的作用。结果:经甲状腺素和(或)胰岛素诱导后能够分化出少突胶质细胞,不同浓度T3和(或)胰岛素对少突胶质细胞分化的影响不同。结论:甲状腺素和胰岛素能诱导神经干细胞向少突胶质细胞分化,且促进分化细胞的增殖和成熟,T(340ng/ml)和胰岛素(25μg/ml)共同使用时这一作用较明显。

Objective：To induce neural stem cells （NSCs） differentiating into oligodendrocytes in vitro and provide experiment foundation for NSCs-derived oligodendrocytes. Methods：The primary NSCs were isolated from endbrain and midbrain of neonatal rats, and then the cells were cultured in vitro. NSCs were induced by thyroid hormone（T3） and（or） insulin, and the effect of T3 and insulin was analysed by comparing the proliferation and the differentiation rate of NSCs and by measuring the prominency of GalC-positive cells. Results：NSCs were induced into oligodendrocytes by thyroid hormone and/or insulin,and different concentration of T3 and/or insulin had different effects on differentiation. Conclusion：Thyroid hormone and insulin can induce NSCs to differentiate mainly into oligodendrocyte and promote the proliferation and maturation of oligodendrocytes, The effect is more outstanding when 40ng/ml T3 is used in combination with 25μg/ml insulin.