抗生素诱导小鼠肠道菌群失调对免疫功能和Toll样受体2、4基因表达的影响

Influence of intestinal flora dysregulation induced by antibiotic on immunity and toll-like receptor 2,4 gene expression in mice

目的:探讨抗生素诱导小鼠肠道菌群失调后对机体免疫功能和Toll样受体2、4的影响。方法:60只BALB/c小鼠分为2组:抗生素组和对照组,抗生素组连续7天口服头孢哌酮,7天后经口给予白色念珠菌。结果:抗生素组小鼠肠道内肠杆菌、肠球菌、双歧杆菌、乳酸杆菌较对照组减少,白色念珠菌增加;脾指数、PHA诱导的淋巴细胞转化率、迟发型变态反应、抗体生成细胞数和脾脏的TLR2和TLR4基因表达水平均低于对照组。结论:抗生素诱导肠道菌群失调后小鼠免疫功能和TLR2、TLR4 mRNA表达均降低。

Objective：To study the effects of intestinal flora dysregulation induced by antibiotic on immune function and tolllike receptor （TLR） 2,4. Methods：Sixty BALB/c mice were divided into two groups：antibiotic and control group. The mice in antibiotic group were treated with cefoperazone orally for consecutive 7 days. After 7 days,mice were inoculated with Candida albicans. Results：The quantity of Enterobacteriaceae,Enterococcus, Bifidobacterium and Lactobacillus in antibiotic therapy group was significantly lower than that in the control group. The quantity of Candida albicans increased in the antibiotic therapy group. Spleen index,lymphocytic transformation rate induced by PHA, delayed-type hypersensitivity and the number of antibody secreting cells, the gene expression of TLR2 and TLR4 mRNA in spleen were lower than those in the control group. Conclusions：The intestinal flora dysregnlation induced by antibiotic may decrease the immune function and toll-like receptor 2,4 gene expression in mice.