从金银花中提取绿原酸的工艺研究

Study on Extracting Process of Chlorogenic Acid from Flos Lonicerae

用不同浓度的乙醇从金银花中提取绿原酸,以正交实验进行优化工艺研究,采用紫外分光光度法测定提取液中绿原酸的含量,探讨了乙醇浓度、乙醇倍量、回流时间、pH值、浸泡温度对绿原酸含量的影响。实验结果表明,最佳的工艺条件是:10倍量50%乙醇为浸提剂,浸泡温度为70℃,pH=7,回流时间为1h,提取次数为2次。

The extracting process of Chlorogenic acid from Flos Lonicerae was studied with different concentrations of ethanol. The processes were then optimized by orthogonal test, and Chlorogenic acid was determined by UV. The effects of concentration of ethanol, folds of ethanol, refluxing time, pH and extracting temperature on Chlorogenic acid content were studied. The result showed that the optimum extracting technology of Chlorogenie acid from Flos Lonicerae includes the extraction conditions of 10 folds of 50% ethanol , dip in 70℃ water , pH = 7 , refluxing for 2 times and 1 h each time.