抑癌基因PTEN在宫颈癌变过程中的表达及意义

Expression of PTEN in Carcinogenesis Process of Cervical Cancer and its Significance

[目的]探讨PTEN在宫颈癌变中的作用。[方法]应用免疫组织化学技术(SP法)检测抑癌基因PTEN在32例正常子宫颈上皮、158例宫颈上皮内瘤变及64例宫颈浸润癌组织中的表达。[结果]32例正常宫颈组织中,PTEN阳性表达率为93.8%,56例CINⅠ、102例CINⅡ和CINⅢ及64例宫颈浸润癌,其PTEN阳性表达率分别为75%、58.8%和40.6%。在从正常宫颈上皮到上皮内瘤变、宫颈癌的癌变过程中,其组织中PTEN的表达呈进行性降低(P<0.05)。[结论]PTEN表达降低是宫颈癌变过程中的早期事件,也是宫颈组织恶变的信号。检测PTEN有助于了解宫颈癌变的生物学行为。

[Purpose] To explore the role of PTEN in carcinogenesis process of the cervical cancer. [Methods] The expression of PTEN in 32 eases with normal epithelium, 158 cases with cervical intraepithelial neoplasia（CIN） and 64 eases with cervical carcinomas was detected by immunohistochemical S-P technique with anti-PTEN antibody. [Results] The positive rate of PTEN expression in 32 cases with normal cervical epithelium, 56 cases with CINⅠ , 102 cases with CINⅡ and Ⅲ, and 64 cases with cervical carcinoma was 93.8%, 75.0%, 58.8% and 40.6%, respectively. PTEN expression reduced progressively in carcinogenesis process from normal cervical epithelium to cervical intraepithelial neoplasia to cervical carcinoma. [Conclusion] Reduced expression of PTEN is an early event in cervical carcinogenesis, and maybe the signal of cervical malignant change, The detection of PTEN expression is helpful tor understanding the biological behavior of cervical carcinogenesis.