CP-MS法与ASS法测量全血Fe、Cu、Pb的比较和分析

The comparison of Mircro-elemental Analysis in Blood By ICP-MS and ASS

目的比较原子吸收光谱法(ASS)与电感耦合等离子体质谱(ICP-MS)检测全血中的微量元素(Fe、Cu、Pb)的相关性和一致性,评价电感耦合等离子体质谱(ICP-MS)检测全血Fe、Cu、Pb的可靠性。方法随机抽取临床全血标本,分别以原子吸收光谱法及电感耦合等离子体质谱(ICP-MS)法同时检测全血Fe、Cu、Pb。测量结果进行统计学分析,对比两种方法的相关性、一致性。结果电感耦合等离子体质谱(ICP-MS)与原子吸收光谱法(ASS)测全血Fe、Cu、Pb总体均数的差异性无统计学意义(P>0.05),呈现强相关性(相关系数r分别为0.999、0.997、0.999);批内、批间重复性试验结果无显著性差异,且精密度符合要求(CV<5%)。但当样品浓度较高(或较低)时,两种方法测量的一致性较差。结论电感耦合等离子体质谱(ICP-MS)在全血标本微量元素检测中,相对原子光谱法,具有样品需求量少、线性范围宽、分析速度快、能同时分析多种微量元素等优点。

Objective To compare to the correlation and consistency of the method of ICP-MS and ASS in testing of mioro-elemental analysis in blood, such as the level of Fe, Cu and Pb, and evaluate the reliability of the method of ICP-MS using in the clinical laboratory. Methods Collecting more than 40 clinical samples of blood by random, to analyze the level of Fe, Cu and Pb according to the instructions of the instrument of Agilent 7500a ICM-MS, AAnalyst 700 ASS, and the appropriate standard of the trade. The obtained results of two groups, we compared the correlation and consistency using the statistical method. Results There are no differences between those two methods in comparison to the total arithmetic means of the sampling （P〉0.05）, and indicating a strong correlation （ the related coefficient is 0.999,0.997,0.999）.The repetition of the intra-assay and the extra-assay are both meet to the requires, and no differences existing. However, when the level of the testing elemental of specimens are relatively very high or low, the obtained results of the two instruments are not consistent. Conclusions There are many advantages to use the ICP-MS method analyzing the Mircro-elemental in the blood, such as its high speed of analyzing process, broad linear range, and the little volumes of sampling. Compared to the method of ASS, the ICP-MS instruments can analyze more than one kind of Mircro-elemental, and it is more convenient to the clinical laboratory.