温度变化对刺参同工酶的影响

Effects of Temperature Changes on Isozymes in the Sea Cucumber,Apostichopus japonicus

采用同工酶电泳方法,对在不同温度处理下刺参(Apostichopus japonicus)的乳酸脱氢酶(LDH)、酯酶(EST)和超氧化歧化酶(SOD)的表达情况进行分析。本文结果显示刺参组织内无LDH同工酶的表达。在10℃到20℃的温度突变处理中,12 h内EST和SOD均有新增酶带,在20℃到10℃的温度突变1 h内EST表达有新增酶带。3 h内SOD表达有新增酶带。在不同变温幅度的处理中,EST和SOD只有1条酶带。这表明,刺参对温度突变可产生积极的响应,对于其适应环境温度的变化具有重要意义。

The effects of acute temperature change and diel fluctuating temperature on the isozyme patterns of LDH, EST and SOD of the sea cucumber Apostichopus japonicus were studied. In the experiment of acute temperature change, young A. japonicus were subjected to heat exposure （from 10 to 20 ℃ ） and cold exposure （from 20 to 10 ℃）, respectively. The samples were taken at 0 h, 1 h, 3 h, 12 h, 24 h and 72 h after heat or cold exposure, respectively. In the experiment of diel fluctuating temperatures, young A. japonicus were subjected to four temperature treatments （18 ℃, 18 ± 2 ℃, 18± 4 ℃ and 18 ± 6 ℃）. The results showed that there was no expression of LDH isozyme. In the experiment of acute temperature changes, within 12 h heat exposure, there were new isomers that appeared both in EST and SOD. After 1 h cold exposure, there was one new band EST2 in the EST isozyme pattern. After 3 h cold exposure there were two new bands band S2 and band S3 in the SOD isozyme pattern. In the experiment of diel fluctuating temperature, there were no changes in the patterns of EST and SOD isozymes.