不同海拔高度木荷种群遗传多样性的ISSR分析

ISSR analysis of genetic diversity of Schima superba populations at different altitudes

利用ISSR分子标记技术分析了浙江天台山不同海拔高度木荷种群的遗传多样性和遗传分化。用12个引物对4个木荷种群共80个样品进行扩增,共检测到170个位点,其中多态位点154个,多态位点百分率(P)为90.59%。木荷总的Shannon信息指数(I)为0.5033,Nei指数(h)为0.3408,表明种水平的遗传多样性较高。而种群水平的遗传多样性比种水平低,P平均为63.68%,I平均为0.3789,h平均为0.2608。AMOVA分子差异分析表明,在总的遗传变异中,29.56%的变异存在于种群间,70.44%的变异存在于种群内,种群间的基因分化系数(Gst)为0.2348。木荷种群间的基因流为1.6293,4个种群间的平均遗传距离为0.1500。

In this paper, Schima superba populations were collected from different altitudes at Tiantai Mountain in Zhejiang Province, and their genetic diversity and genetic differentiation were analyzed by using inter simple sequence repeat （ISSR） molecular technique. ISSR amplification was conducted with 12 primers for 80 individuals from g populations, and 154 polymorphic loci were detected from total 170 loci, with the percentage of polymorphic loci （P） being 90. 59%. The Shannon＇ s information index （I） and Nei＇s gene diversity （h） of S. superba at species level were 0. 5033 and 0. 3408, respectively, indicating a high genetic diversity of S. superba at species level, while the genetic diversity of S. superba at population level was lower than that at species level, with the mean values of P, I and h being 63.68% , 0. 3789 and 0. 2608, respectively. Molecular variance （AMOVA） analysis demonstrated that the among-population component accounted for 29.56% of the total variation, while the within-population component accounted for 70. d4%, the genetic differentiation coefficient （Gst） among the populations being 0. 2348. The gene flow among the populations was 1. 6293, and the mean pair-wise genetic distance was 0. 1500.