丹酚酸B体外干预骨髓间充质干细胞分化过程中cTnT的表达

Expression of cTnT induced by Salvianolic Acid B in differentiation of bone marrow Mesenchymal Stem Cells into myocardium-like cell

[目的]观察丹酚酸B(SalB)体外干预骨髓间充质干细胞(MSCs)后细胞形态及心肌特异性蛋白肌钙蛋白T(cTnT)的表达。[方法]培养、纯化及鉴定MSCs,用SalB、5-氮胞苷组(5-aza)、SalB联合5-aza(SalB+5-aza)分别定向诱导分化,观察此过程中MSCs的形态学变化并采用免疫细胞化学法鉴定诱导后MSCs心肌特异性肌钙蛋白T(cTnT)的表达。[结果]诱导后的MSCs体积增大,增殖减慢,并出现肌管样结构;免疫细胞化学结果显示诱导后MSCs表达心肌特异性蛋白cTnT。[结论]SalB在体外定向诱导MSCs分化为心肌细胞的过程中发挥了一定的作用。

[Objective] To observe the effect of salvianolic acid B（SalB） in differentiation of bone marrow mesenchymal stem cells （MSCs） into myocardium-like cells. [Methods] MSCs was cultured, purified and evaluated, then MSCs was induced by 5-aza, SalB and 5-aza associating SalB to observe the cells＇ morphology change and evaluate the expression of cardiac troponin T （cTnT） by immunocytochemistry method. [Results] After induction, the volume of MSCs increased, the proliferation speed decreased. Myotube-like structure appeared and expressed cTnT. [Conclusions] SalB plays a certain role in the process of induction into myocardimn-like ceils.