化学性迷路破坏动物模型及前庭代偿过程的定量观察

Animal model of chemical labyrinth destruction and quantitative observations on vestibular compensation.

目的探讨建立化学性迷路破坏豚鼠模型的方法及内耳的组织病理学改变。方法动物全麻后行中耳注射氯仿和石腊油混合液（０．１ｍｌ～０．２ｍｌ），出现单侧前庭功能丧失症状后，分别存活４８ｈ、１周和１月，处死后颞骨火棉胶包埋制片，标准光镜下观察。结果各存活期动物内耳均有不同程度损害。结论根据功能及形态学的改变提出判定此动物模型成功的四项标准。并通过对动物自发性眼震、头偏斜及失衡行为的定量观察，客观评价了前庭代偿的动态变化过程。

Objective To establish the model of chemical labyrinth destruction and observe the histopathology of chloroform induced injury of inner ear in guinea pigs. Methods The right ears of the animals were injected with 50% solution of chloroform and oil(0.1 ml￣0.2 ml).After the appearence of unilateral vestibular deficits,the animals were permitted to survive for 48 hours, 1 week and 1 month respectively.Histopathology of the animals' inner ears was observed. Results It was found that the inner ears were damaged at all survival time and the damage was severer as time went on.The damage of the cochlea was severer than that of vestibule, the saccule was severer than the utricle, ampulla and crista. Conclusion Four standards judging the successful model of labyrinth destruction were put forward according to the functional and morphological changes.Dynamic changes of vestibular compensation were evaluated by quantitative observations on slow phase eye velocity(SPEV) and frequency of spontaneous nystagmus(SN), head deviation(HD) and postural asymmetries (PA) appeared following acute loss of vestibular function.