正加速度对大鼠肾功能和组织结构的影响

Effect of +G Z on renal function and structure in rats.

目的观察加速度（＋ＧＺ）作用后血清中尿素氮（ＢＵＮ）、肌酐（Ｃｒ）、碱性磷酸酶（ＡＫＰ）、β２－微球蛋白（β２－Ｍ）和Ｃａ２＋浓度及肾脏病理改变。方法４８只雄性大鼠随机分为４组，每组１２只。地面未受附加＋ＧＺ暴露为Ａ组，受＋１ＧＺ为Ｂ组，＋１０ＧＺ为Ｃ组和Ｄ组。Ｂ组与Ｃ组在第３次加速度作用（每次３ｍｉｎ，间隔４０ｍｉｎ，共３次）后１０ｍｉｎ处死。Ｄ组在２４ｈ后处死，测上述指标。结果以Ｂ组为对照组，Ｃ组ＢＵＮ有显著增高（Ｐ＜０．０１）。Ｄ组Ｃａ２＋、β２－Ｍ有明显升高（Ｐ＜０．０５）。ＢＵＮ是恢复至正常最快的肾功能指标。受＋１０ＧＺ重复作用后，光镜下见肾脏血管呈收缩改变，电镜下见肾小球及肾小管的细胞、线粒体有病理改变。结论＋１０ＧＺ的重复暴露对大鼠肾脏功能和结构都有损害。

Objective To observe harmful effect of +G Z on renal function and structure in rats. Methods Forty eight male Wistar rats were randomly divided into four groups( n =12 for each group). Group A served as ground control. Group B were repeatedly exposed to +1 G Z while group C and Group D exposed to +10 G Z for 3 min in animal centrifuge (3 times, with 40 min interval in between). Blood samples of group B and group C were collected 10 min after +G Z exposure, and those of group D collected 24 h after exposure, for blood urea nitrogen (BUN), creatinine (Cr), alkaline phosphetase(AKP), Ca 2+ and β 2 microglobulin (β 2 M) examination. At the same time,of kidney were taken for microscopic and electron microscopic examination. Results In comparision with group A, BUN,Cr, AKP,and Ca 2+ levels in group B were significantly elevated, while β 2 M remained unchanged. In comparison with group B, BUN in group C, and β 2 M, Ca 2+ in group D were significantly increased ( P <0.05). In microscopic examination vasoconstriction was observed, and in electron microscopic examination pathological changes of ultrastructure and mitochondria of glomeruli and renal tubules were found in rats after repeated +10 G Z exposure. Conclusion Repeated +10 G Z exposure may exert harmful effects on renal function and structure in rats. The elevated BUN following repeated +10 G Z exposure restore to normal within 24 h.