抗凝因子测定影响因素的探讨

Approach of the effect in determination of throbosis factors (Abstract)

本文观察了不同温度，不同时间，不同采血条件对保存的血浆中抗凝血酶－Ⅲ（ＡＴ－Ⅲ）、蛋白Ｃ（ＰＣ）、蛋白Ｓ（ＰＳ）的活性影响。结果显示蛋白Ｃ活性最不稳定，在３２℃条件下，硅化管与普通玻璃管保存血浆２４小时（ｈ）其活性分别只相当于即刻的５５％和４２％，在室温（２０℃）、冰箱（４℃、－２０℃）硅化管与玻璃管保存２４ｈ，则分别为６９％和５６％，７６％和８０％，８７％和８７％。ＡＴ－Ⅲ、ＰＳ对温度变化比较稳定，但随着保存时间的延长活性也有逐渐下降的趋势，２０℃，２ｈ、６ｈ、２４ｈ，ＡＴ－Ⅲ、ＰＳ活性分别为即刻的９５％、８７％、８０％；９１％、８１％、７７％。ＡＴ－Ⅲ在－２０℃保存４８ｈ稳定性较好，为１０１％；ＰＳ在－２０℃，４８ｈ活性为即刻检测结果的８５％。

$$$$ Zhao Ainong,et al Affiliated School of Chinese Medical UniversityAbstract The activities of AT Ⅲ,PC,PS in serum are observed under different conditions(temperature,time,and storing method).The results show:PC is the least stable factor.At 32℃,the activity of AT-Ⅲ stored in silicatube and glass tube decreases to55% and 42% arter 24h.At 20℃,it falls to 76%,80%;at4c,it decreases to 76%,80%;at-20℃,it decreases to 87%,87%.AT Ⅲ,PS are stable to temperature. But being stored for 2h,6h,24h,at 20c,it falls to 95%,87%,80%;PS’s activity falls to 91%,81%,77%,AT 20℃ within 48h AT Ⅲ and PS are stable.