雌激素受体α、β亚型在大鼠睑板腺组织的表达

Expression of estrogen receptor alpha and beta in meibomian gland tissue of rats

目的探讨雌激素受体亚型(ERα、ERβ)在正常雌性大鼠睑板腺组织的分布。方法22只SD雌性大鼠颈椎脱臼处死,取上眼睑睑板组织,常规石蜡包埋,连续切片;采用免疫组织化学方法显示ERα、ERβ在睑板腺组织的表达,并设正常大鼠子宫作阳性对照;PBS代替一抗作阴性对照。同时采用放射免疫分析方法检测大鼠血清雌二醇的浓度。结果ERα和ERβ在大鼠睑板腺腺泡的基底细胞、过渡细胞和成熟细胞有表达。在22只大鼠中,有21只(95·45%)ERα在基底细胞呈阳性表达,有15只(68·18%)ERα在过渡细胞和成熟细胞呈阳性表达;有14只(63·64%)ERβ在基底细胞呈阳性表达,有8只(36·36%)ERβ在过渡细胞和成熟细胞呈阳性表达。ERα阳性表达率明显高于ERβ,统计学分析有显著性差异(P<0·05)。正常大鼠血清雌二醇含量为22·13±3·54pg/ml。结论大鼠睑板腺腺泡上皮有ERα和ERβ表达,以ERα表达占优势;ERα和ERβ定位在细胞质和细胞核。

Objective To explore distribution of the estrogen receptor alpha（ERa）and estrogen receptor beta（ERβ）in meibomian gland tissue of the normal female rats. Methods 22 SD female rats were killed by dislocation of cervical vertebra, the tarsal plate tissues of the upper eyelids were in paraffin imbedding and made to a series of sections which were then studied by means of immunohistochemical method, and the uterus of rats was used as positive control and PBS as negative control. The serum estradiol level of rats was detected by radioimmunoassay. Resaits ERα and ERβ were expressed in acinar epithelia of meibomian gland,s. Among 22 rats, 21 rats （95.4%） were found to be ERα positive expression in the hasa cell of the meibomian gland aeinar, 15 rats （68.2%） were found to be ERα positive expres sion in transitional cell and mature cell of the meibomian gland acinar. 14 rats （63. 6%） were found to be ERβ positive expression in the basa cell of the meibomian gland aeinar, 8 rats （36. 4%） were found to be ERβ positive expression in transitional cell and mature cell of the meibornian gland aeinar. The positive staining rate for ERα was higher than that for ERβ, which have statistically significant difference （P 〈0. 05）. The serum estradiol level of rat was 22. 13±3. 54pg/ml. Conclusion ERα and ERβ positively expressed in the acinar epithelia of meibornian gland of rats with a superior expression of ERα, and both ERα and ERβ were located in eytoplasma and nuclear. The present results provided the datum of molecular biology for studying the effects of estrogens on the meibomian gland.