颈椎后纵韧带胶原、蛋白多糖、钙含量变化的研究

Study on diversification of quantity of collagen and proteoglycan and calcium in the posterior longitudinal ligament of the human cervical spine

[目的]通过定量测定颈椎后纵韧带总胶原、Ⅰ型胶原、Ⅱ型胶原、蛋白多糖和钙含量改变,探讨颈椎后纵韧带在颈椎病发病机理中的意义。[方法]收集脊髓型颈椎病下位颈椎后纵韧带15例作试验组,同年龄段正常下位颈椎后纵韧带10例作对照,Weossner法测定两组总胶原含量;酶联免疫吸附法测定Ⅰ型、Ⅱ型胶原含量;间苯三酚分光光度法测定蛋白多糖含量;甲基百里香酚蓝比色法测定钙含量;进行HE染色和Masson染色,对比观察后纵韧带的显微结构变化特点。[结果]试验组总胶原、Ⅰ型胶原和蛋白多糖含量低于对照组;试验组Ⅱ型胶原含量较对照组高;试验组Ⅰ型/Ⅱ型胶原比值低于对照组;试验组钙含量较对照组高,组间对比均有统计学意义(P<0.05);病理结构显示试验组弹力纤维、胶原纤维肿胀,排列紊乱。[结论]颈椎病患者颈椎后纵韧带退变,总胶原、Ⅰ型胶原、Ⅱ型胶原、蛋白多糖和钙代谢发生紊乱,颈椎后纵韧带分子水平的生物化学变化与颈椎病发生发展有密切关系。

[ Objective] To study the mechanism of posterior longitudinal ligament in cervical spine through measuring the collagen and Proteoglycan and Calcium changes. [ Method ] Fifteen specimens of cervical longitudinal ligament from cervical spondylotic myelopathy （CSM） and ten control specimens from corpses without cervical spondylosis were obtained. The content of the collagen was measured by Weossner method. Collagen type Ⅰ and Ⅱ were measured by Enzyme-Linked Immunosorbentassay （ELISA） method. Phloroglucinol spectrophotometer to determine the change of amount of Proteoglycan. The Calcium by Methyl-Thymes-Blue （MTB） Colorimetric Method. The specimens were treated hy Hematoxylin and Eosin （HE） stain method and by Masson stain method, the pathological changes of two groups were observed through microscopy. [ Result] In CSM, as compared to the control groups, there showed a decrease in the contents of the total Collagen, Collagen type Ⅰ and Proteoglycan, and increase in the content of collagen type Ⅱ. The rate of type Ⅰ/Ⅱ in CSM was lower than that in control groups. An increase in the content of the Calcium. All of which have statistic significance （ P 〈 0. 05 ）. The disorder of elastic fabric and collagen fabric were observed. [ Conclusion] In CSM, the cervical longitudinal ligaments degenerated and its diversification of biochemistry remained in disorder. CSM is notably associated with the degenerated cervical longitudinal ligaments.