表皮生长因子受体3和4在喉鳞状细胞癌中的表达及意义

Expression and significance of ErbB3 and ErbB4 in patients with laryngeal squamous cell carcinomas

目的:探讨表皮生长因子受体3(ErbB3)与ErbB4基因在喉鳞状细胞癌中的表达及其与喉癌生物学、临床病理特征的关系。方法:采用RT-PCR法,分别对36例喉鳞状细胞癌患者的癌组织、14例切缘正常黏膜及11例良性增生性病变中ErbB3与ErbB4基因的表达进行检测。结果:在喉癌与良性增生性病变中,ErbB3 mRNA过度表达阳性率分别为66.7%和18.2%(P<0.01);ErbB4 mRNA过度表达阳性率分别为25.0%和9.1%(P>0.05)。ErbB3和ErbB4在喉癌组织与切缘正常黏膜中的表达差异均无统计学意义(均P>0.05)。ErbB3与ErbB4的表达与喉癌的临床病理特征无显著相关。结论:ErbB3与ErbB4在喉癌的发生、发展中具有不同的作用,在切缘正常黏膜中的过度表达对肿瘤复发可能起着一定的作用。

Objective：To explore expression of ErbB3 and ErbB4 genes in laryngeal squamous cell carcinomas and their relation with the biological and clinicopatbological parameters. Method： Expression of ErbB3 and ErbB4 mRNA in 36 cases of laryngeal carcinomas and normal mucosa of incisal margin, 11 cases of benign proliferative lesions were examined by reverse transcription polymerase chain reaction （RT-PCR）. Result.-In laryngeal carcinomas and benign proliferative lesions,over-expressive positive ratioes of ErbB3 were 66. 7%,18. 2% respectively （ P 〈0. 01）. However, that of ErbB4 were 25.0%, 9.1% respectively（ P 〉0.05）. Differences of expressive level of ErbB3 and ErbB4 were not significant between laryngeal carcinoma and normal mucous of incisal margin（ P 〉0.05）. In addition, expressive level of ErbB3 and ErbB4 were not associated with diversity of clinical pathologic characters（ P 〉0.05）. Conelnsion：ErbB3 and ErbB4 genes play different role in carcinogenesis and development, and relate to the reoccurrence of carcinoma.