苦参碱对肝癌患者肿瘤浸润淋巴细胞的影响

Effects of matrine on tumor-infiltrating lymphocytes of patients with hepatocellular carcinoma

目的:研究不同浓度苦参碱对肝癌患者肿瘤浸润淋巴细胞(TIL)增殖、体外杀瘤活性的影响.方法:将苦参碱分为0.1,0.2,0.4,0.6,0.8g/L5个浓度组,分别加入含有IL-2的DMEM培养基,体外诱导扩增TIL,通过MTT法检测TIL增殖、体外杀瘤活性,采用流式细胞术测定T淋巴细胞亚群,研究苦参碱体外对肿瘤免疫功能的影响.结果:苦参碱低浓度组(终浓度分别为0.1,0.2g/L)对TIL作用不明显,高浓度组(终浓度为0.8g/L)能抑制TIL增殖和体外杀瘤活性(P<0.01),中浓度组(终浓度分别为0.4,0.6g/L)能促进TIL增殖和体外杀瘤活性(P<0.01),CD8+数量有所增加,CD4+/CD8+比值有所下降(P<0.01).结论:合理利用苦参碱可增强患者肿瘤免疫能力.

AIM： To explore the effect of different concentra- tions of matrine on proliferation and activity of tumor-infiltrating lymphocytes （TIL） of patients with hepatocellular carcinoma in vitro. METEODS： Different concentrations of matrine （0. 1, 0.2, 0.4, 0.6, 0.8 g/L） were respectively added into DMEM medium supplemented with IL-2. The effect of matrine on proli- feration and activity of TIL in vitro was studied by MTF, and the Phenotype was detected by flow cytometry （FCM）. RESULTS： The lower concentration of matrine （0.1,0.2 g/L） had no effect on TIL. High concentration of matrine （0.8 g/L） had a remar- kable inhibitory effect on proliferation and antitumor activity of TIL （P 〈0.01 ）. The middle concentration of matrine （0.4, 0.6 g/L） enhanced the proliferation and antitumor activity of TIL in v/tro （ P 〈 0. 01 ）. The percentage of CD8^＋ increased and the ratio of CD4^＋/CD8^＋ decreased （ P 〈 0.01 ）. CONCLUSION ： The reasonable use of matrine could enhance the patients＇ specific antitumor immune function.