血红素氧化酶-1在协调性异种心脏移植中的作用

Role of HO-1 in concordant cardiac xenotransplantation

目的:探讨血红素氧化酶-1在协调性异种心脏移植中的作用及其机制.方法:建立金黄地鼠对大鼠异种心脏移植模型.随机将实验动物分为4组:对照组、环孢素组、脾切除组、脾切除+环孢素组.观察异种移植物的生存时间.HE染色观察各组移植物组织病理学改变.免疫组化染色观察各实验组不同时间移植物组织中NF-κB,P-selectin,HO-1的表达情况.结果:脾切除组移植物生存时间较对照组和环孢素组延长.脾切除联用环孢素较其它组移植物生存时间延长.移植心脏发生排斥反应时,移植物组织中NF-κB,P-selectin表达呈阳性.长期存活的移植心脏组织中HO-1表达阳性,未见到NF-κB,P-selectin表达.结论:异种移植物血管内皮细胞HO-1表达上调对移植物长期存活有一定意义,对移植物血管内皮细胞具有一定的保护作用.

AIM： To investigate the role of Heme oxygenase-1 （HO-1） in the protection of xenograft endothelium and its mechanism. METHODS： Hamster to rat cardiac transplantation model was constructed. The animals were divided into 4 groups randomly： control group, cyclospofine A （CsA） group, splenectomy group, splenectomy ＋ CsA group. Hamster heart was transplanted into rat abdominal cavity. The survival time of the xenografts were monitored. The histological changes of the xenogafts were investi- gated by HE staining. H0-1, NF-KB and P-selectin expressions were detected immunohistochemically in the xenografts of different groups. RESULTS： The survival time of the xenografts in the splenectomy group was longer than that in the control group and CsA group. The survival time of the xenograft in the splenec- tomy ＋ CsA group was prolonged significantly as compared with the other group. NF-KB and P-selectin expressions were observed in the rejected xenografts. H0-1 expression was positive in the endothelial cells of long-term survived xenografts in the splenecto- my ＋ CsA group. However, no evidence of NF-KB and P-selectin expression was discovered. CONCLUSION： Upregulation of HO-1 can protect endothelium of the xenograft, which is vital to the long-term survival of the xenograft.