温度对淡紫拟青霉NH-PL-03菌株培养特性及毒力的影响

Effect of temperature on Paecilomyces lilacinus culture characteristics and its toxicity to Ditylenchus destructor.

试验测定了淡紫拟青霉NH-PL-03菌株在不同温度条件(15～35℃)下培养过程中生长特性(OD值、pH值、菌丝干重、孢子量、基质消耗)的变化趋势及对线虫的致死率,系统研究温度对淡紫拟青霉生长的影响,结果表明:温度不仅影响菌体的生长,且影响次生代谢物的毒力。在15～30℃范围内,菌体生长、孢子生成及基质消耗(C、N)速率随温度升高而升高;25℃为菌体生长的最合适温度,培养第5d进入快速增长期,第9d菌丝干重达到1.08g,第10d菌液孢子量达到401.2万个/mL;30℃时总体毒力最高,第7d培养液对茎线虫的致死率达到64.60%,其次是25℃和20℃,最高致死率分别达到56.17%和37.21%;15℃最高致死率仅为24.71%。说明在一定范围内,温度上升有利于毒力产物生成。

In this paper, the effect of temperature on Paecilomyces lilacinus growth was investigated. The growth index for OI）,pH,mycelia dry weight, and spore number and their toxic effect on nematodes at different temperatures （15 -35℃ ）were analyzed. Results show that temperature is a key factor of fermentation. Paecilomyces lilacinus exhibits normal growth within 20- 30b ,optimum mycelia growth and sporulation occur at 25b, mycelia biomass reaches 1.08g in 9 days, spore number reaches 4,012 × 10^6/mL in 10 days. Between 15 - 30℃ , higher temperatures can increase C and N consumption and toxin production. At 30℃ , cultured 7d, the toxicity to Ditylenchus destructor can reach 64,60 %.