启动条件对活性污泥变形菌群落的影响

Effect of Start-up Conditions on Proteobacteria Community Structure

为研究生物反应器启动条件对于变形菌(proteobacteria)群落的影响规律,分别利用针对变形菌α与β亚纲的特异性16S rDNA引物,通过变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)方法,对两个序批式反应器(sequencing batch reactor,SBR)的变形菌群落进行分析.实验中采用不同来源的驯化污泥在相同进水条件下进行启动,同时模拟了城市污水碳源浓度条件对于变形菌类两个亚纲种群结构的影响.结果表明,不同污泥类型经相同启动条件的驯化产生了特异性的微生态结构和功能表现,碳源的投加对于菌群结构影响较小,但显著改变了脱氮效率.

Denaturing gradient gel electrophoresis （DGGE） method were used to investigate the influence of start-up conditions on proteobacteria community in two sequencing batch reactors with specific 16S rDNA primers for α-and β-subdivision proteobacteria. Two kinds of sludge were used for incubation during the start-up of two reactors. The effect of carbon source on proteobacteria community structure was also studied. The result shows that different sludge type results in specific community structure and functional performance. Carbon source obviously affects efficiency of nitrogen removal, but the community structure remains relatively stable.