低温保存动脉的粘弹性力学评价指标的初步建立

Viscoelastic Evaluating Guideline for Cryopreserved Arteries

目的建立低温保存动脉的粘弹性力学评价指标。方法使用动态力学分析仪(DMA-2980,TA In-struments,New Castle,Delaware,USA)比较研究不同低温保存方案下动脉蠕变行为与新鲜对照组的差异。结果低温保存动脉相对新鲜对照组而言,其粘弹性均有损失;随降温速率的增加(1.5,5,10℃/min),其粘弹性损失趋于增加,即1.5℃/min为本研究中最佳降温速率。

Objective To establish a new evaluating guideline for clinical applications of cryopreserved arteries. Methods The creep behaviors of the cryopreserved arteries were observed with different cryopreservation methods by using a dynamic mechanical analyzer （DMA-2980, TA Instruments, New Castle, Delaware, USA）, Results The viscoelasticity of cryopreserved arteries was apparently less than that of the fresh group,and it means that cryopreservation was sure to damage the viscoelasticity of arteries to a certain extent; the loss of viscoelasticity of the cryopreserved arteries increased along with the increase of cooling rates from 1.5, 5, through 10 ℃/min, and it was obvious that 1.5 ℃/min was the optimal cooling rate for all the cryopreservation methods used in the present study. Conclusion The degree of accordance of the creep behaviors of cryopreserved arteries with the theoretical models set up in this study could be used for evaluating the viscoelasticity of cryopreserved arteries; the remained viscoelasticity of cryopreserved arteries could be used as a novel valuating guideline for optimization of the cryopreservation processes.