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TOLL样受体-4和髓样分化蛋白-2在血吸虫病小鼠肝脏的表达及意义 被引量：1

Expression and Significance of Toll-like Receptor 4 and Myeloid Differentiation 2 in the Liver of Rats with Schistosomal Cirrhosis
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摘要 目的:探讨血吸虫病小鼠肝脏TOLL样受体-4(TLR-4)和髓样分化蛋白-2(MD-2)的表达及其在肝脏损害中的作用。方法:将40只健康小鼠随机分成正常对照组(N组,20只)与模型组(M组,20只),M组采用腹部敷贴法制作血吸虫病模型,造模150天后处死动物,鲎试验法测定血清内毒素水平;免疫组化和RT-PCR方法检测肝脏中TLR-4和MD-2的表达。结果:M组血清内毒素水平高于N组(P<0.01);M组小鼠肝脏TLR-4/MD-2表达水平高于N组(P<0.01);M组小鼠肝脏TLR-4/MD-2高表达主要位于肝窦内皮细胞及肝窦区周围细胞。结论:血吸虫病肝硬化可发生肠源性内毒素易位、内毒素血症;脂多糖通过其信号转导蛋白TLR-4和MD-2在肝脏的高表达,激活一系列促炎基因的转录,可能是引起血吸虫病肝脏损害的原因之一。 Objective:To explore the expression and significance of Toll-like receptor 4(TLR-4)and myeloid differentiation 2(MD-2)in the liver of rats with schistosomal cirrhosis.Method:Subdividing 40 rats into 2 groups:group N(control),group M(model).The M group included 20 rats infected percutaneously with cercariae of schistosomiasis japonica.Immunohistochemistry and RT-PCR were applied to detect the protein and mRNA expression of TLR-4/MD-2,vena cava blood was tested for endotoxin.Results:The expression of TLR-4/MD-2 in the liver in M group was significantly higher than N group(P<0.01),the level of endotoxin in M group was significantly higher than N group(P<0.01).The expression of TLR-4/MD-2 was mainly at liver sinusoidal endothelial cell and the cell around the liver sinuses.Conclusion:Endotoxin translocations and high expression of TLR-4/MD-2 play an important role in lesion of liver function of rats with schistosomal cirrhosis.
作者 李岽健 杨镇 牛丽文 张爱龙 肖亮
机构地区 华中科技大学同济医学院附属同济医院综合科
出处 《微循环学杂志》 2007年第3期7-9,F0002,共4页 Chinese Journal of Microcirculation
基金 湖北省重大传染性疾病防治研究科技专项项目(编号:2004AA306A)
关键词 髓样分化蛋白-2 TOLL样受体-4 血吸虫病 小鼠肝脏 肠源性内毒素血症 信号传导蛋白 肝脏损害 内源性感染 Schistosomal cirrhosis Endotoxemia TLR-4/MD-2 Rats
分类号 R392.11 [医药卫生—免疫学]
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参考文献6

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二级参考文献19

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共引文献14

同被引文献27

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微循环学杂志
2007年 第3期

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