摘要
目的:观察兔颈动脉粥样硬化斑块形成过程中血管平滑肌细胞(VSMC)功能的动态变化。方法:高脂喂养加气体干燥术建立兔颈动脉内膜损伤模型,将实验动物分为正常对照组;高脂饲料加气体干燥术后1月组、2月组、3月组。用免疫组织化学法检测各组的平滑肌肌动蛋白(浕-SM-actin)、细胞凋亡和Ki-67核抗原(Ki-67)、基质金属蛋白酶-2(MMP-2)和组织型基质金属蛋白酶抑制物-2(TIMP-2)表达情况。结果:正常对照组无浕-SM-actin、凋亡和Ki-67表达,MMP-2和TIMP-2低水平表达。高脂饲料加气体干燥术后浕-SM-actin、细胞凋亡和Ki-67、MMP-2和TIMP-2表达增高。浕-SM-actin阳性细胞主要为一些胞核呈椭圆形或圆形的平滑肌细胞及一些泡沫细胞,且随着胞浆的空泡化,MMP-2和TIMP-2表达密度下降;部分泡沫细胞Ki-67阳性,细胞凋亡表达阳性。结论:VSMC和平滑肌细胞源性泡沫细胞为高脂饲料加气体干燥术后形成的动脉粥样硬化斑块的主要成分。MMP-2和TIMP-2主要在平滑肌源性泡沫细胞中表达,且随着胞浆的空泡化,MMP-2和TIMP-2表达密度下降。斑块中的平滑肌源性泡沫细胞不仅可以发生凋亡,也可表现出一定的增殖活性。
Objective:To investigate the function of vascular smooth muscle cell(VSMC)in rabbit carotid artery injured by airing drying.Method:To establish rabbit carotid artery injured model by using airing drying and high cholesterol and lipoid diet,36 animals were divided into normal diet group and injured group,the expression of ɑ-smooth muscle actin(ɑ-SM-actin),apoptosis and proliferation,matrix metalloproteinase-2(MMP-2)and tissue inhibitor of matrix metaloproteinase-2(TIMP-2)were evaluated by immunohistochemistry.Results:There is no ɑ-SM-actin、Ki-67 nuclear antigen(Ki-67)and apoptosis expression in the carotid of normal diet group and MMP-2 and TIMP-2 expression were low.All of their expression elevated in the injured group.ɑ-SM-actin positive cells are VSMCs and foam cells and with the grow of the foam cell,MMP-2 and TIMP-2 expression were decrease.Foam cells can both apoptosis and proliferate.Conclusion:The major components of the atherosclerosis plaque caused by airing drying and high cholesterol and lipoid diet are VSMC and VSMC derived foam cell.During the formation of VSMC derived foam cell from VSMC,it can express MMP-2 and TIMP-2,which is the major source of MMP-2 and TIMP-2,with the grow of the foam cell,MMP-2 and TIMP-2 expression were decrease.During the formation of VSMC derived foam cell from VSMC,they can apoptosis and proliferate.
出处
《微循环学杂志》
2007年第3期35-38,F0004,共5页
Chinese Journal of Microcirculation
