人ICAM-1真核表达重组体在QSG7701细胞中的表达

Expression of recombinant of intercellular adhesion molecule-1 (ICAM-1) cDNA in QSG7701 cells

目的探讨建立真核表达重组体pcDNA3.1HisB-ICAM-1稳定转染细胞株。方法分子克隆构建真核表达重组体pcDNA3.1HisB-ICAM-1,脂质体转染将重组质粒导入QSG7701细胞,G418筛选,对稳定转染的细胞克隆进行RT-PCR,并对转染的细胞进行免疫组织化学染色。结果ICAM-1表达重组体在转染细胞中可稳定表达,而且以2μg含量表达率最高。结论人ICAM-1真核表达重组体在转染细胞QSG7701中可高表达,为研究ICAM-1的生物学作用和功能奠定基础。

[Objective] To build human ICAM-1 stable expression cell strain.[Methods] Eukaryotic expression recombinant pcDNA3.1hisB-ICAM-1 needed to be constructed;next expression recombinant plasmid was transfected into QSG7701 cell line by using lipofectamine and the stable transfected cell line was selected in medium containing geneticin（G418）.The result of reverse transcription PCR of the transfected cell line.[Results] The recombinant pcDNA3.1hisB-ICAM-1 transfected QSG7701 cell and stable expressive on the transfected cell line,and the most expression in 2 μg.[Conclusion] Stable expression cell strain of human ICAM-1 is built.