半乳糖化白蛋白磁性阿霉素纳米粒的急性毒理实验研究

Study of acute toxicity of galactosed human serum albumin magnetic adriamycin nanoparticle for rats

目的研究半乳糖化白蛋白磁性阿霉素纳米粒经外周静脉用药的药物毒性。方法将受试动物分为8个剂量组,观察静脉给药后的急性毒性试验,采用Bliss法计算LD50,统计分析其对大鼠的外周血细胞和生化指标的影响。给予腹腔注射3次后,再皮下注射,观察其有无过敏现象发生。结果半乳糖化白蛋白磁性阿霉素纳米粒的动物半数致死量为515.5mg/kg,同时肝、肾功能的损害和心肌酶学的改变也明显减轻。20只动物进行过敏实验,观察到一只动物有过敏现象。结论半乳糖化白蛋白磁性阿霉素纳米粒是一种很好的新型改进性药物,它经过修饰后,明显的减轻了药物对心脏,肾脏和心脏的毒性损害作用。有望批量生产,在临床上推广试用。

[Objective] To study the acute toxicity of galactosed human serum albumin magnetic adriamycin nanoparticle（GAMANP）for rats by injected in vein.[Methods] 8 teams were given corresponding dose of GAMANP in vein,then detecting median lethal dose（LD50）of the tow drugs with Bliss method.Observing blood biochemical changes of the rats and the pathological changes of organs of the rats.Observing the irritability of the GAMANP,and observing the difference of the tow kinds drug by the SPSS.[Results] The LD50 of GAMANP was 515.5 mg/kg to rat.GAMANP effectively reduced the changes of the blood biochemistry and pathological damage such as cardiotoxicity,hepatotoxicity,nephrotoxicity and gastroenterotoxicity.The spleen remained the same in both.We observed the anaphylaxis on one of the 20 rats.[Conclusion] GAMANP significantly diminished the tox（icity of adriamycin in the important organs of the body.The LD50 of GAMANP was improved greatly than that of ADM.It is a new type of anticancer drug in the foreground in the future.