聚甲基丙烯酰吗啉枝化壳聚糖梳型聚合物的pH敏感性及其对辅酶A的控制释放

pH-sensitivity of combed copolymer P(methylacrylmorpholine) branched chitosan and its controlled delivery on coenzyme A

利用马来酸酐单酰化壳聚糖（CS-Ma）与甲基丙烯酰吗啉（MAMP）共聚,合成了一种新型梳型聚合物-聚甲基丙烯酰吗啉枝化壳聚糖[P（CS-Ma-MAMP）],并用FTIR、H1-NMR对其结构进行表征,聚合物的分子量用凝胶渗透色谱（GPC）测定。用UV研究了其对辅酶A（Co A）的控制释放,结果表明在24℃、pH=6.8时,随着聚合物水溶液浓度的增大,对辅酶A的控制量就增多;pH在3.7～9之间变化时,可以通过调节聚合物水溶液的pH实现对辅酶A的控制释放,并初步探讨了其控制释放的机理。

A kind of pH-sensitive polymer P（CS-Ma-MAMP） was prepared by Chitosan（CS）,maleic anhydride（Ma） and Methylacrylmorpholine（MAMP） with grafting and copolymerization.The copolymer obtained was analyzed by FT-IR and ^1H-NMR,and the molecular weight and polydispersity were determined by gel permeation chromatography（GPC）.The results of UV analysis showed that at 24℃ and pH 6.8 solution,the adsorption of Co A increased with the concentration of the copolymer solution increasing,or at 24℃ and pH between 3.7 and 9 values,the copolymer can well control delivery on coenzyme A by changing the pH of the copolymer solution.The release mechanism of coenzyme A was also investigated.The results suggested that the copolymer could be used as drug delivery system.