HBV-DNA定量与HBV-M检测结果的对比研究

Comparison of Detection of HBV-DNA with Routine Detection of HBV-M

目的:了解HBV-DNA定量检测与HBV血清标志物定性或定量检测结果之间的关系。方法:运用荧光定量PCR(FQ-PCR)检测患者血清中的HBV-DNA,同时运用ELISA法或时间分辨荧光免疫法检测HBV血清标志物,并对结果进行对比研究。结果:HBsAg(+)HBeAg(+)HBcAb(+)组患者血清HBV-DNA检出率最高为94.62%(176/186),HBsAg(+)HBcAb(+)组患者血清HBV-DNA检出率为42.86%(30/70),HBsAg(+)HBeAb(+)HBcAb(+)组患者血清HBV-DNA检出率为26.17%(56/214),HBsAg(+)HBeAg(+)HBcAb(+)组显著高于其它各组(P<0.01)。结论:血清HBV-DNA水平与HBV血清标志物的表现模式有关,HBeAg与HBV-DNA有高度的相关性。HBV-DNA与HBV-M结合能全面了解HBV感染、复制及传染性,也为指导治疗提供客观依据。

Objective：To explore the relationship between detection of HBV-DNA and HBV serum markers, Methods：HBV-DNA was detected with fluorescent quantitative PCR （FQ-PCR） and HBV was routinely determined with ELISA or time-resolved immunofluorometric assay, Results：The detecting rate of HBV-DNA was 94.62%（176/186） in patients of HBsAg （＋）HBeAg（＋）HBcAb （＋）,42,86%（30/70） in patients of HBsAg（＋）HBcAb（＋）,26.17%（56/214） in patients of HBsAg（＋）HBeAb（＋）HBcAb（＋）, The parameter was significantly higher in HBsAg（＋）HBeAg（＋）HBcAb（＋） than in others（P 〈0,01）.Conclusion：There is a correlation between HBV-DNA and HBV-M, espccialy between HbeAg and HBV-DNA ,Both HBV-DNA and HBV-M determination could reflect the infection、replication of HBV and provide objective evidences for diagnosis and treatment monitoring of hepatitis B.