兔膝关节持续被动运动仪的研究及应用

The development and application of a continuous passive motion instrument

目的通过对兔全层关节软骨缺损修复的研究,验证持续被动运动(CPM)实验仪器的实用性和稳定性。方法将20个兔膝关节建立全层关节软骨缺损动物模型,随机分为A、B两组,A组术后给予CPM干预,B组仅笼内自由活动。CPM干预4周后,处死兔并取其全层关节软骨行HE染色和大体形态学评分。结果A组全层关节软骨缺损修复的效果优于B组,差异有统计学意义(P<0.05)。结论CPM实验仪器设计合理、结构简单实用、稳定性好,适用于兔膝关节进行CPM的动物实验。

Objective To validate if the continuous passive motion instrument we developed is practical and stable for animal experiment. Methods Ten New Zealand male rabbits were used to establish the animal model of full- thickness defect of articular cartilage in the facies patellaris of femur with their both knees. The rabbits were then randomly divided into groups A and B （ 10 in each group）. The rabbits in group A were administered with CPM of both knees daily for 4 weeks, while those in group B remained normal cage activity only. At the end of the treatment, all the animals were sacrificed, and their articular cartilage was harvested for HE staining and observation. Results It was found that group A had a significantly better repair of the full-thickness defects of articular cartilage than that of the group B, as reflected by the range of motion and morphological observation of the knees （ P 〈 0.05）. Conclusion The CPM instrument we de- signed is reliable with regard to its stability of performance and suitable for animal experiment.