慢性砷暴露对小鼠肾组织DNA损伤及牛磺酸和维生素C的保护作用

Preventive effects of taurine and vitamin C on mice kidney DNA damages exposed to arsenium chronically

目的探讨砷对肾组织DNA氧化性损伤以及牛磺酸和维生素C的保护作用。方法小鼠60只,随机分为6组,即:低剂量组(1mg/LAs2O3)、中剂量组(2mg/LAs2O3)、高剂量组(4mg/LAs2O3)、牛磺酸保护组(4mg/LAs2O3+150mg/kg牛磺酸)、维生素C保护组(4mg/LAs2O3+45mg/kg维生素C)以及生理盐水对照组,用HE染色法进行组织病理学观察,用免疫组化检测肾组织内8-OH-dG的水平。结果各染砷组小鼠肾组织出现不同程度细胞肿胀,胞浆空泡变性等病理学改变。免疫组化表明砷暴露组8-OH-dG的表达显著增高。牛磺酸和维生素C保护组肾组织病理学变化较轻,8-OH-dG表达较少(P<0.01)。结论慢性砷暴露可引起小鼠肾组织DNA氧化性损伤和病理变化,牛磺酸和维生素C具有保护作用。

Objective To evaluate the protective effects of taurine and vitamin C on arsenium（As） exposed mice kidney. Methods 60 mice were divided into 6 groups, low dose group （1 mg/L Arsenic trioxide（As2O3））, medium dose group（2 mg/L As203 ）, high dose group （4 mg/L As2O3 ）, taurine protective group （4 mg/L As2O3 ＋ 150 mg/kg taurine）, vitamin C protective group （4 mg/L As2O3 ＋ 45 mg/kg vitamin C）, and normal saline group as control. Kidney tissues were taken and performed with HE staining and 8 hydroxy-2＇-deoxyguanine expression were examined by immunohistochemistry. Results The kidney cells in As treated groups showed swelling and vaeuolation. 8-OH-dG were also strongly expressed in As exposed groups. Two prophylactic groups showed very mild damages with very low 8-OH-dG level（ P 〈 0.01 ）. Conclusions The kidneys showed severe pathologic injuries and DNA oxidative damages after long-term As exposure. Taurine and vitamin C alleviated the damage significantly.