急性化脓性脑膜炎早期诊断的实验研究

Early diagnosis of acute pyogenic meningitis.

目的建立免疫斑点法检测脑膜炎双球菌(A群)、肺炎球菌抗原,探讨应用免疫斑点法对早期快速诊断细茵性脑膜炎的应用价值。方法建立该实验体系,以A(λ)值(取对数Log)为纵坐标,抗原浓度(取对数Log)为横坐标,绘制标准曲线,求出回归方程。结果最小脑膜炎球菌抗原检出量为36 ng/ml。最小肺炎球茵抗原检出量为13.4 ng/ml。结论初步建立了用免疫斑点法检测急性化脓性脑膜炎中脑膜炎双球菌(A群)、肺炎球菌抗原的诊断方法,具有灵敏度高、特异性强,简便、快捷,不需特殊仪器,结果可保存等优点,非常适于在广大临床实验室推广应用。

Objective To define the application value of dot immunolinding assay in diagnosing bacterial menilggitis,by determining the known antigens of Neisseria meningitidis A-group and Streptococcus pneumoniae diagnostic approach for the acute pyogenic meningitis. Methods Based on the data obtained and the analysis of the variation of absorbana in relation to the content of antigen, a standard curve can be plotted for deriving the regression equation and correlation coemcient. Results The minimum detectable quantity of antigen was 36 ng/ml. The minimum detectable quantity of antigen is 13.4 ng/ml. Conclusion The sensitivity and specificity of the method is high, and easy to use without the need to use any special instrument. Furthermore, the results obtained can be stored. It proves to be a method that is suitable for extensive clinic applications.