基质金属蛋白酶-9基因多态性与乙型肝炎肝硬化的关系

Polymorphisms of matrix metalloproteinase-9 in hepatitis B-related cirrhosis

目的探讨基质金属蛋白酶(matrix metallo proteinase,MMP)-9基因启动子区多态性与乙型肝炎肝硬化的关系。方法应用PCR法和限制性片段长度多态性分析方法分别检测100例乙型肝炎肝硬化患者和124名正常对照者的MMP-9-1562C/T多态性,分析其基因型与乙型肝炎肝硬化发病风险相关性。结果MMP-9-1562C/T多态性的基因型及等位基因频率在乙型肝炎肝硬化组和对照组间的分布差异无统计学意义(P>0.05)。结论MMP-9启动子1562位点基因多态性与乙型肝炎肝硬化易感无明显相关。

Objective The aim of this study was to probe the relationship of polymorphisms at position-1562 in the promoter region of the matrix metalloproteinase （MMP）-9 gene to the occurrence of HBV-related liver cirrhosis. Methods Restriction fragment length polymorphism PCR was used to study polymorphisms of MMP-9-1562C/T in 100 patients with HBV-related liver cirrhosis and in 124 healthy persons served as control. Results There were three kinds of genotypes at position -1562 of MMP-9, namely, CC genotype（435bp）, CT genotype（435bp, 247bp, 188bp） and TT genotype （247bp, 188bp）. No statistical significance was found at the distribution of the three genotypes and allele frequencies between HBV-related liver cirrhosis group and control group（P〉0.05）. C/C, C/T and T/T genotype frequencies in patients were 70%, 24%and 6%, respectively, and those in control group were 69.35%, 24.2%and 6.49%（P〉0.50）. C and T allele frequencies of MMP-9 were 84% ,16% in patients, and 80.90/00,19.1% in control group（P=0. 916）, Adjus- ted OR value was 0. 970 （95%CI：0. 431-2. 182）. Conclusion There is no significant association between gene polymorphisms at position-1562 in the MMP-9 promoter and susceptibility of HBV-related liver cirrhosis.