荧光定量PCR技术检测vgb基因在棉花中的表达

Expression Level of Detecting vgb Gene by Fluorescence Real-time Quantitative PCR in Cotton

应用荧光定量PCR技术SYBR Green I荧光染料法,对透明颤菌血红蛋白基因vgb在转基因棉花中的表达水平进行相对定量分析,建立了快速检测转基因产品中基因表达量的方法。通过该方法检测出了vgb基因在转基因棉花不同株系里的相对表达量,且表达量有差异。该方法具有灵敏度高、重复性好、定量准确、速度快等特点。

The real-time quantitative reverse transcriptase polymerase chain reaction （RT-PCR） for rapid specific and sensitive measurements of transforing growth mRNA expression was established with dsDNA-binding dye SYBR Green Ⅰ. Through this method, the relative expression level of vgb gene in a different transgenic cotton is detected,and the different expression is detected in different plant lines of trangenic cotton. This technology has the characteristics of strong peculiar high sensitivity, fine repetitiveness and accurateness, fast speed. A rapid detection of the expression of the exogenous gene technology GMOS was established.