卡瓦胡椒及胡椒的荧光AFLP分子标记研究

Studies on the AFLP Molecular Markers of Kava and Piper nigrum

对6份卡瓦胡椒材料、23份栽培胡椒和野生胡椒材料、1份不同属的草胡椒材料、1份不同科的普通烟材料,共计31份试验材料,进行荧光AFLP分子标记,研究卡瓦胡椒与胡椒及其他近缘野生种的亲缘关系。结果表明,在相似系数0.52处将31份种质划分为5个类群,第1个类群有普通烟,第2个类群有草胡椒,第3个类群是卡瓦胡椒,第4个类群有胡椒,第5个类群有山胡椒、蒌叶和蒟蒌。说明卡瓦胡椒与胡椒及其他近缘野生种的亲缘关系有一定距离,为卡瓦胡椒真伪性的分子鉴定和构建卡瓦胡椒的指纹图谱提供了基础性的工作。

The genetic relationship between Kava and Piper nigrum has been studied by the AFLP molecular markers. 31 test materials were used in the paper, including 6 Kava genotypes, 23 cultivated and wild peppers, 1 Peperomia pellucida kunth and 1 tobacco. Based on the fluorescence-labeled AFLP, the 31 germplasms were derided into 5 different groups at 0.52 of the genetic similarity coefficient. The result showed that the genetic distance between Kava and other Piper germplasms was far, and the fluorescence-labeled AFLP was applicable to the identification and DNA fingerprint construction of Kava.