沙漠乔桑离体快繁与试管苗生根基质的研究

Study on Rapid Tissue Culture and Culture Medium of Tube Seedling to the Desert Tall Mulberry

用常规组织培养方法对沙漠乔桑待萌发冬芽、萌发侧芽进行外植体消毒,以MS(Murashige和Skoog,1962)作基本培养基,添加不同质量浓度的BA(6-苄基腺嘌呤)、NAA(萘乙酸),配制不同激素组合的培养基,筛选出最适宜的诱导、增殖和生根培养基配方。然后,以蛭石代替琼脂设计生根培养基质、加糖与不加糖、灭菌与不灭菌等不同的培养方案,进行试管苗扦插生根的试验研究。研究结果表明:最适宜的桑芽诱导萌发的培养基为MS+1.0mg/LBA+0.1mg/L NAA;快速繁殖的培养基为MS+0.5mg/LBA+0.05mg/L NAA;试管苗的生根培养基可以用蛭石代替琼脂,不加糖、不灭菌,添加0.2mg/L的NAA,试管苗的生根与琼脂培养基没有差别,且能提高移栽成活率、降低成本。研究结果可为沙漠乔桑的工厂化生产提供借鉴。

The convention tissue culture method was treated to sprouting hibernaculum and lateral bud of the desert tall mulberry after exophyte disinfection.Using the basic culture medium with MS（Murashige ＆ stoog,1962）which adding the different concentration of BA and NAA,to made the different compounds hormone combination culture medium.Then selected the most appropriate medium for induction multiply and taking root.Then replaced the agar of vermiculite to design root taking culture medium.With sugar and non-sugar,sterilization and non-sterilization,conducted the experimental study of test tube seeding cuttage for taking root.The result indicated that：the most suitable culture medium of bud spronting was MS＋1.0mg/LBA＋0.1mg/LNAA and the most suitable culture medium of fast reproduction was MS＋0.5mg/LBA＋0.05 mg/LNAA.When replaced agar of vermiculite with adding no-suger and without antiseptic.The rate of taking root was not different with agar culture medium.It was also a good way of enhancing transplant survival rate and reducing cost.It could providing the model for the desert tall mulberry factorization production.