高效液相色谱法测定复方对乙酰氨基酚片的含量

Quantitative determination the content of compound paracetamol tablets by HPLC

目的建立复方对乙酰氨基酚片中对乙酰氨基酚、咖啡因、乙酰水杨酸含量的HPLC测定方法。方法采用高效液相色谱法,以甲醇-0.1%二乙胺-冰醋酸(40∶60∶4)为流动相,检测波长275nm,流速1.0 mL.min-1。结果对乙酰氨基酚、咖啡因、乙酰水杨酸的线性范围分别为25～400μg.mL-1(r=0.9999),6～120μg.mL-1(r=1),18.4～736μg.mL-1(r=1);平均回收率分别为99.64%(RSD=0.14%)、99.94%(RSD=0.22%)、100.84%(RSD=0.30%)(n=6)。结论该方法分离度良好,灵敏度高,准确且简单易行。

OBJECTIVE To establish a method for the quantitative determination of compound paracetamol tablets by HPLC. METHODS A HPLC was developed for the compound paracetamol tablets, the mobile phase consisted of methanol -0. 1% diethylamine -acetic acid glacial （40： 60： 4）, the detection wavelength was 275nm and the flow rate was 1.0 mL · min^ - 1. RESULTS The linear range of paracetamal was 25 - 40μg · mL^- 1 （ r = 0. 9999 ）, that of caffeine was 6 - 120μg · mL^-1（ r = 1 ）and aspirin was 18.4 - 736μg · mL^-1（ r = 1 ）. The average recovery of the three components were 99.64% （RSD =0. 14% ） ,99.94% （RSD =0. 22% ） and 100.84% （RSD =0. 30% ） （n = 6） respectirely. CONCLUSION The proposed method is detached degree, highly sensitive, accurate and simple.