我国6个绵(山)羊群体遗传分化的微卫星分析

Genetic diversity of microsatellite DNA in six breeds of China sheep(goat)

为进一步了解我国绵(山)羊群体的品种特性及其遗传分化,本文利用微卫星标记对我国6个绵(山)羊群体遗传分化进行了分析。采用中心产区典型群随机抽样方法用聚丙烯酰胺凝胶电泳检测乌珠穆沁羊7个微卫星位点,并引用同实验室小尾寒羊、滩羊、湖羊、同羊、长江三角洲白山羊(参照群体)的相关资料进行群体遗传分化水平分析。研究表明:7个微卫星位点在乌珠穆沁羊、小尾寒羊、滩羊、湖羊、同羊、山羊这6个品种中均存在遗传多态性,各座位等位基因均较丰富。根据标准遗传距离、DA遗传距离以及模糊相容关系进行聚类分析,湖羊与同羊首先聚为一类,乌珠穆沁羊和小尾寒羊聚为一类,然后与滩羊聚为一类,5个绵羊品种最后与山羊相聚。

In order to investigate the breed characteristics and genetic diversity of China sheep （goat） , microsatellite markers were used in the present study for 6 breeds of china sheep （goat）. Gene frequencies of 7 microsatellite loci of 123 Ujumuqin Sheep were examined by using the method of random sampling in typical colonies of the central area of the habitat, and the PCR amplifying and the PAGE electrophoresis. Relational data were cited to analysis the genetic differentiation of Ujumuqin sheep, Han sheep, Tan sheep, Hu sheep, Tong sheep and White Goat of Yangtze Triangle District. The results indicated there were genetic polymorphisms at seven microsatellite markers in these six sheep （goat） breeds. Analysis of the relations between six populations of sheep and goat based on standard genetic distance, DA genetic distance and fuzzy similarity relations, Hu sheep and Tong sheep clustered first, Ujumuqin sheep and Han sheep and then with Tan sheep, five sheep populations clusteredd with goat at last.