红头兰(Tuberolabium quisumbingii)胚培养和快速繁殖

Embryo Culture and Rapid Propagation of Tuberolabium quisumbingii

以红头兰的胚为材料,研究了红头兰的胚培养和快速繁殖技术.结果表明:培养基中无机盐的浓度和激素影响胚的萌发,在1/4MS培养基加入0.5mg/L的KT效果最佳;培养基中加入椰子水、土豆提取物、蛋白胨和活性炭均有利于胚的萌发;在继代培养中,适宜的培养基为1/3MS+6-BA2mg/L+NAA0.01mg/L+椰子水10%+蛋白胨2g/L+活性炭2g/L+蔗糖20g/L+琼脂7g/L;分化和壮苗培养基为1/2MS+NAA0.5mg/L+IBA0.5mg/L+蔗糖20.0g/L+AC2.0g/L+蛋白胨2.0g/L+琼脂7g/L。

In this paper the embryo culture and rapid propagation of Tuberolabium quisumbingii was researched. The results show that the concentration of major salts and the phytohormones in MS medium affect the germinative capacity of the embryos and the suitable medium is 1/4 MS ＋ KT 0.5 mg/L; it is all conductive to germination of embryos to add coconut water, peptone, potato and activated charcoal in MS medium; 1/3 MS ＋ 6- BA 2 mg/L ＋ NAA 0.01 mg/L＋ coconut water 10% ＋ peptone 2 g/L ＋ AC 2 g/L ＋ sucrose 20 g/L ＋ agar 7 g/L is the optimum medium for subculture; 1/2 MS ＋ NAA 0.5 mg/L ＋ IBA 0.5 mg/L ＋ sucrose 20.0 g/L ＋ AC 2.0 g/L ＋ peptone 2.0 g/L ＋ agar 7 g/L is the optimum medium for differentiation and plant establishment.