喜树果渣多糖的微波提取工艺研究

Technology of Extracting Polysaccharides in Fructus Remnant of Camptotheca acuminata with Microwave

应用均匀设计法及一系列单因素选择试验优化了喜树多糖的微波提取工艺条件,比较了蒸煮回流法和微波提取法提取喜树多糖的效率.喜树多糖的微波提取优化条件是:固液比为0.1(1∶10),NaOH浓度1.55 mol.L-1,微波功率284 W,微波作用时间9.2 min.采取2次浸泡,2次微波处理方式,提取液的浓缩比为5,乙醇加入量为30 mL,沉淀放置48 h.

The extracting of extracting polysaccharides in fructus remnant of Camptotheca acuminata between the traditional method and microwave was compared, and the condition of extracting polysaccharides in fructus remnant of C. acuminata with microwave was optimized, by single factor experiment. The optimal conditions of extracting polysaccharides were 1：10 ratio of material to the liquid, 1.55 mol · L^-1 NaOH,and 9.2 min microwave irradiation（284 W）for 2 times, the concentrated scale is 5, 30 mL ethanol was added, and polysaccharide deposited for 48 h.