摘要
目的:探讨氩氦刀术后肺癌细胞凋亡及其可能机制。方法:A549细胞爬片后行氩氦刀冷冻,末端标记法(TUNEL)原位检测细胞凋亡,DNA电泳观察梯带,免疫组织化学SABC法检测bc1-2和bax表达。结果:在冷冻周边区,光镜形态学检查及TUNEL染色可见细胞凋亡,DNA电泳可见有梯带出现。免疫组化显示,bcl-2蛋白表达在冷冻前后无明显变化,bax蛋白在冷冻后其表达上调。结论:氩氦刀可以引起冷冻区周边部分细胞发生凋亡,其凋亡机制有bax蛋白参与,而与bcl-2无关。为增加冷冻疗效,术后可结合放化疗。
Objective:To investigate the apoptosis and its possible mechanism of human A549 cells induced by argon -helium -based CRYOcare System in vitro. Methods:The A549 cells cultured on the cover glass were subject to cryo - treatment via CRYOcare Cryosurgical Unit. Then cell apoptosis was examined with terminal deoxynucleotidyl transferase -mediated dUTP nick end labeling(TUNEL) and DNA gel electrophores. The expression of bcl -2 protein and bax protein was detected by immunohistochemical procedures. Results: In the periphery of the cryolesion, apopotic cells can be determined by morphological analysis,TUNEL staining and DNA ladder. The expression of bax protein increased significantly,meanwhile the expression of bcl - 2 protein had no obvious change. Conclusion: The argon - helium - based CRYOcare System can induce cell apoptosis in the periphery of the cryolesion by upregulation of bax expression but not bcl - 2 expression. And chemotherapy or radiotherapy can be combined to improve the cryosurgery efficiency.
出处
《现代肿瘤医学》
CAS
2007年第8期1055-1057,共3页
Journal of Modern Oncology
基金
全军"十一.五"医药卫生科研项目(06MB021
关键词
氩氦刀
冷冻
凋亡
argon - helium
cryosurgery
apoptosis