非小细胞肺癌组织中bcl-2和mt-p53蛋白表达与血管生成的关系及其意义

The relationship of bcl-2,mt-p53protein expression and angiogenesis and their significance in non-small cell lung cancer

目的:探讨非小细胞肺癌组织(NSCLC)中bcl-2及mt-p53蛋白表达与血管生成的关系。方法:采用免疫组化s-p法检测60例NSCLC标本中bcl-2、mt-p53蛋白表达,以血管内皮细胞表面抗原CD34单抗标记测定MVD。结果:在NSCLC中bcl-2阳性率31.67%,bcl-2阳性率与分化程度有关(P<0.05),mt-p53表达阳性率为48.33%;MVD以及mt-p53表达与NSCLC的淋巴转移(P<0.05)有关,MVD低表达者术后生存期明显长于高表达组(P<0.01);bcl-2阳性组病人中mt-p53阳性率明显低于bcl-2阴性组(P<0.05),bcl-2阳性组MVD明显低于bcl-2表达阴性组(P<0.05),相反,mt-p53阳性组MVD高于阴性组(P<0.01);在NSCLC中,在bcl-2阴性病人中mt-p53(+)组MVD(21.39±6.35)与mt-p53(-)组之间的MVD(15.91±5.68)存在显著性差异(P<0.01),但在bcl-2阳性病人中二组之间无显著差异(P>0.05)。结论:NSCLCbcl-2基因高表达可能与肿瘤血管生成的抑制有关,mt-p53基因则可能通过下调bcl-2促进肿瘤血管生成。

Objective ：To study the relationship between the expression of bcl -2 , mt -p53protein and angiogenesis in non -small cell lung cancer （NSCLC） tissue. Methods： The expression of bcl -2 and mt -p53were measured by s - p immunohistochemical method in 60 cases of human NSCLC, meanwhile, anti - CD34 monoclonal antibody directed against endothelial marker was used to assay MVD. Results： The rate of bcl -2 expression was 31.67% , significantly correlated with differentiation of non - small cell lung cancer （ P 〈 0. 05 ）. The rate of mt - p53expression was 48.33%. MVD and mt - p53significantly associated with lymph node metastasis （ P 〈 0.05 ）. The prognosis for patients with high MVD was significantly poorer than that of patients with low MVD（ P 〈 0.01 ）. The mt - p53 positive rate in bcl - 2 - negative group was higher than that of bcl - 2 - positive group, bcl - 2 and mt - p53 protein expression had correlation （ P 〈 0.05 ）. Patients with bcl - 2 - negative had significantly higher MVD than those with bcl - 2 - positive ones （ P 〈 0.05 ） . On the contrary, patients with mt - p52 positive had significantly higher MVD than those with mt - p53 negative ones （ P 〈 0.01 ）. In bcl - 2 - negative patients, MVD of cases with mt- p53 - positve was significantly higher than that of cases with mt - p53 - negative（ P 〈 0.05 ） . But in bcl - 2 - positive patients , the MVD of cases with mt - p53 - positve was similar to that of cases with mt - p53 - negative. Conclusion： Bcl -2 may inhibit angiogenesis, mt -p53 could down regulate bcl -2 to promote angiogenesis.