猪Myopalladin基因多态性与胴体性状的关联分析

Analysis on Associations of Myopalladin Gene Polymorphisms with Carcass Traits in Pigs

采用RT-PCR扩增与直接测序,对猪肌肉组织的一条表达序列标签(EST)(GenBank登录号为BM445313)进行SNP筛查。检测到2个新的SNPs,通过BLAST在NCBI非冗余数据库中进行同源性比对,发现该EST位于Myopalladin基因的3′UTR区。在猪参考家系SCAU-LL F2群体中对其中的一个SNP进行Csp6I-RFLP基因分型,并与胴体瘦肉率和皮脂率进行关联分析,结果表明基因型CT与CC的瘦肉率均值分别为46%和45%、皮脂率均值分别为42%和44%,在瘦肉率和皮脂率上的差异均达到了显著水平(P<0.05)。由此可见,猪Myopalladin不同基因型对胴体性状有着重要影响,是猪育种应用中的一个潜在遗传标记。

Single nucleotide polymorphisms （SNPs） were detected by RT PCR amplification and sequencing on the EST （GenBank accession No. BM445313）which was expressed in porcine muscle. Two new SNPs were found and located in the 3＇UTR region of Myopalladin gene by homologous comparative analysis in the non-redundant sequence database of NCBI through BLAST search. One of the SNPs was genotyped by RFLP with restriction enzyme Csp6I and used for as sociation analysis with carcass traits in the SCAU-LL F2 pig resource population. The results showed that the average carcass lean meat percentage of CT and CC genotype were 46 % and 45% respectively, and the average carcass skin ＆ fat percentage of the two genotype were 42% and 44% respectively, these differences were significant （P〈0.05）. Therefore, it could be seen that porcine Myopalladin genotypes had significant influence on carcass traits, and might be considered as a potential genetic marker for selection.