鸡ADSL基因和GARS-AIRS-GART基因对鸡肉肌苷酸(IMP)含量的影响

The Effect of ADSL and GARS-AIRS-GART Genes on Inosine Monophosphate Content in Chicken Meat

以鸡ADSL基因、GARS-AIRS-GART基因为侯选基因,以隐性白羽鸡、丝羽乌骨鸡、萧山鸡、白耳鸡、藏鸡为试验材料,采用PCR-SSCP方法对ADSL外显子2、GARS-AIRS-GART基因5′侧翼序列进行SNPs检测。结果各发现了一个单核苷酸多态性位点。各种基因型与胸肌IMP含量的最小二乘分析结果表明:ADSL基因TT型个体的胸肌肌苷酸含量极显著地高于CC型、显著地高于CT型个体,CT型个体也高于CC型,但差异不显著;GARS-AIRS-GART基因TT型个体的胸肌肌苷酸含量极显著地高于CT型和CC型个体,CT型个体也高于CC型,但差异不显著。ADSL和GARS-AIRS-GART基因的合并基因型对IMP含量也有显著的影响,合并基因型为TTTT的个体IMP含量显著高于CCCC合并基因型的个体,高出1.584 mg/g,因此可以利用该合并基因型对鸡的肉质风味性状进行标记辅助选择。

This study was designed to investigate the effect of ADSL, GARS-AIRS-GART and combination genotypes of ADSL and GARS-AIRS-GART on IMP content in chicken meat. Five chicken breeds, including Recessive White, Silkies, Baier, Tibetan and Xiaoshan chickens were used. The primers for the exon2 in ADSL gene and 5＇UTR region in GARS-AIRS GART gene were designed and the SNPs were detected by PCR-SSCP method and DNA sequencing. Two SNPs were detected in exon2 of ADSL gene and 5＇UTR region of GARS-AIRS-GART gene sepa- rately. The least square analysis showed that TT genotype birds had greatly significant higher IMP content than CC （P〈0. 01） and significant higher than CT （P〈0. 05） genotype birds, TC genotype birds also had a little higher IMP content than CC genotype birds, but the difference was not significant in ADSL gene. TT genotype birds had significant higher IMP content than CC and CT genotype birds （P〈0.05）, TC genotype birds also had a little higher IMP content than CC genotype birds, but the difference was not significant in GARS-AIRS-GART gene. The combination genotypes of ADSL and GARS-AIRS-GART genes also had significant effect on IMP content. The IMP content of the individuals with TTTT combination genotype was 1. 584 mg/g higher than the individuals with CCCC combination genotype. Therefore we putatively drew the conclusion that the combination genotypes of ADSL and GARS-AIRS-GART gene could be used as the molecular genetic marker to select the chicken for meat quality traits.