口服鸭瘟弱毒苗诱导鸭局部黏膜和系统免疫中IgA、IgM和IgG发生规律研究

Regularity of IgA,IgM and IgG Antibodies in Local Mucosal and Systemic Immunity of Ducks Induced by Attenuated Duck Plague Virus Vaccine Strain Vaccinated Orally

为探讨鸭瘟弱毒苗诱导鸭局部黏膜和系统免疫中抗体发生的规律,本研究将鸭瘟病毒Cha株弱毒苗经口免疫20日龄樱桃谷鸭,60 d内定时随机剖杀5只鸭,采集血清、胆汁、气管和消化道(食道、十二指肠、空肠、回肠、盲肠和直肠)分泌液,应用间接ELISA检测抗DPV的IgAI、gM和IgG滴度(以Log10表示)。结果表明:①血清中检测到IgA、IgM和IgG的时间段分别为9-36、3-15和9-60天,滴度由高到低为IgG、IgM和IgA;②胆汁中检测到IgAI、gM和IgG的时间段分别为6-15、9-12和12-36天,滴度由高到低为IgA、IgG和IgM;③气管中检测到IgA、IgM和IgG的时间段分别为6-60、3-12和9-27天,滴度由高到低为IgAI、gM和IgG;④食道中检测到IgAI、gM和IgG的时间段分别为3-60、3-27和9-60天,滴度由高到低为IgA、IgM和IgG;⑤各肠段抗体滴度由高到低及相应检测到的时间段:十二指肠中为IgA(3-60天)I、gM(3-15天)和IgG(9-27天);空肠中为IgA(6-60天)I、gM(6-12天)和IgG(9-36天);回肠中为IgA(9-60天)、IgM(6-9天)和IgG(15-21天);盲肠中为IgA(6-60天)I、gG(12-36天)和IgM(6天);直肠中为IgA(3-60天)I、gG(12-21天)和IgM(6天)。结果显示,鸭瘟弱毒疫苗经口免疫鸭后,IgM和IgG分别是系统免疫中体液免疫的先锋抗体和主要抗体;IgA、IgG和IgM在胆汁中存留时间短;IgA是气管和消化道中的主要抗体。

The regularity of IgA, IgG and IgM antibodies in local mucosal and systemic immunity of ducks induced by attenuated duck plague virus vaccine strain vaccinated orally were studied in this paper. Twenty-day-old Cherry Valley ducklings were vaccinated orally with attenuated duck plague virus Cha strain and every five vaccinated ducklings were killed punctually in 60 days, and DPV-speciflc antibodies were detected in serum, bile and the secretion from trachea and enteron （oesophagus, duodenum, jejunum, ileum, caecum and rectum） by indirect ELISA（results were presents as the log10 geometric mean antibody titer± SD）. The results showed that the order of antibody titers from high to low was：① IgG, IgM and IgA in serum, and could be detected at 9-60, 3-15 and 9-36 days respectively;②IgA, IgG and IgM in bile,and could be detected at 6-15, 12-36 and 9-12 days respectively;③IgA, IgM and IgG in trachea, and could be detected at 6-60, 3-12 and 9-27 days respectively;④IgA, IgM and IgG in oesophagus and could be detected at 3-60, 3-27 and 9-60 days respectively; ⑤The antibody titers in intestines from high to low and the period of time when the antibodys could be detected were respectively as follows： IgA（3-60 days）, IgM（3-15 days）and IGG（9-27 days） in duodenum; IgA（6-60 days）,IgM（6-12 days）and IGG（9-36 days） in jejunum; IgA（9-60 days）,IgM（6-9 days）and IGG（15-21 days） in ileum; IgA（6-60 days）, IGG（12-36 days）and IgM（6 days） in caecum; IgA（3-60 days）,IgG（12-21 days） and IgM（6 days） in rectum. Data presented here indicated that IgM was produced firstly and IgG was the most important antibody in systemic immunity, and IgA, IgG and IgM preserved in the bile to be short, and IgA was the most important antibody in trachea and enteron after the duckling were vaccinated orally with attenuated duck plague virus strain.