雏鸡初级听神经元的原代培养方法

Dissociation and culture of primary auditory neurons in postnatal chicken

报告对出生后10～16d龄鸡初级听神经细胞（PAN）进行原代培养的方法；用倒置相差显微镜和扫描电镜，观察不同培养时间听神经元的生长发育过程与形态学特征。结果显示，培养PAN数量和形态以及存活时间，均可满足体外研究的要求，可作为多种体外研究的材料。表明PAN原代培养方法．扩大了培养材料的来源，为开展内耳体外研究提供了一种新的实验手段。

In the present study,a primary culture of auditory neurons in 10-16-day old chick ens was carried out. We observed the morphologic features of auditory neurons at different cultur ing stages by using phased-contrast and scanning electron microscope, and particularly described their growth and developmental process. The results indicated that a steady and reliable culturing method established here would contribute to the biological research of inner ear in vitro.