PTEN和P53蛋白在子宫内膜癌中的表达及临床意义

Expression of PTEN and P53 protein in endometrial carcinoma and their clinical significance

目的:探讨PTEN和P53蛋白在子宫内膜癌中的表达及临床意义。方法:采用免疫组织化学S-P法检测42例子宫内膜癌、28例子宫内膜增殖症和20例正常增生期子宫内膜组织中PTEN和P53蛋白的表达。结果:与增生期子宫内膜100%表达率相比,PTEN蛋白子宫内膜非典型增生及子宫内膜癌中的阳性表达率逐渐降低,分别为66.7%和53.1%。随着子宫内膜癌临床分期的升高、病理分级的降低及肌层浸润的加深,PTEN蛋白的表达呈逐渐降低的趋势。子宫内膜癌组织中PTEN蛋白的失表达主要局限于子宫内膜样腺癌,占46.9%,10例子宫内膜浆液性腺癌中则未见PTEN的失表达,PTEN失表达在这两组肿瘤间的差异有统计学意义(P=0.001)。P53蛋白过表达在子宫内膜样腺癌中占25%,在子宫内膜浆液性腺癌中为80%,两组间差异有统计学意义(P=0.001)。结论:PTEN的缺失和P53过表达与子宫内膜癌的发生、发展有关,同时预示着子宫内膜癌的不良预后。

Objective：To investigate the expression of PTEN and P53 protein in endometrial carcinoma and their clinical significance. Methods：The expression of protein PTEN and protein P53 was detected by immunohistochemical staining S-P method in 20 cases of the proliferative phase of endometfium, 28 cases of endometrial hyperplasia and 42 cases of endometfial carcinoma. Results： Compared the positive expression rate of 100% in the normal endometrium, the expression of PTEN was reduced gradually in the endometrial hyperplasia and endometfial carcinoma（66. 7% and 53. 1% , respectively）. In endometfial carcinoma, with progression of clinical stage, decreasing of histological grade and deepening of cancer invasion, the expression of PTEN decreased. The lack of expression of protein PTEN mainly existed in endometfioid adenocarcinoma, which accounted for 46.9%. Wheras, in 10 serous carcinoma cases, the lack of expression of protein PTEN was not seen. The lack of expression of protin PTEN was significant different between these two groups. The over-expression of protein P53 covered 25% in endometrioid adenocarcinoma while covered 80% in serous carcinoma. The differences between these two groups were significant. Conclusion：The expressions of protein PTEN and protein P53 are relevant to the occurrence of endometrial carcinoma. The low expression of protein PTEN and the over-expression of protein P53 in endometrial carcinoma may have relationship with bad prognosis.