胃喜康煎剂对乙酸致胃溃疡大鼠细胞生长因子的影响

Study of Effect of Weixikang Apozema on Growth Factor of Acetic Acid Gastric Ulcer Rats

目的:观察胃喜康煎剂对乙酸致胃溃疡大鼠细胞生长因子的影响。方法:大鼠随机分为空白对照组(简称空白组)、模型组、雷尼替丁组、胃喜康低剂量组(简称低剂量组)、胃喜康高剂量组(简称高剂量组)5组,每组8只。造模、采集标本。放射免疫法测定表皮生长因子(EGF)、转化生长因子-α(TGF-α)含量。免疫组化检测EGF、TGF-α、表皮生长因子受体(EGFR)蛋白表达。结果:胃喜康低剂量组、高剂量组EGF、TGF-α含量增加(P<0.05);EGF、TGF-α、EGFR蛋白表达增加(P<0.05)。结论:胃喜康可能通过激活EGF、TGF-α/EGFR系统而发挥抗溃疡作用。

Objective： To observe the effect of Weixikang apozema on growth factor of acetic acid gastric ulcer rats. Methods： The rats were divided into blank control group （abbreviated as blank group）, model group, ranitidine group, Weixikang low dosage group （abbreviated as low dosage group） and Weixikang high dosage group （ab- breviated as high dosage group） at random, each group has 8 rats. The content of epidermal growth factor （EGF） and transforming growth factor-α （TGF-α） were measured with radioimmunoassay. The protein expression of EGF, TGF-α and EGFR were measured with immunohistochemical method. Results： The content of EGF and TGF-α and the protein expression of EGF, TGF-α and EGFR in low dosage group and high dosage group have increased （P 〈 0.05）. Conclusion：The anti-ulcer function of Weixikang apozema was possibly related to activation of the system of EGF, TGF-α/EGFR.