p27、β-catenin、p21-ras在乳腺导管上皮良、恶性病变中的表达及意义

The expression and significance of p27,β-catenin,p21-ras in breast benign and maligant lesions

目的:探讨p27、β-catenin及p21-ras在乳腺导管上皮良、恶性病变中的表达及意义。方法:应用免疫组化(Envision)法检测乳腺导管上皮一般性增生(15例)、乳腺导管上皮不典型增生(28例)及乳腺浸润性导管癌(25例)组织中p27、β-catenin、p21-ras的表达情况。结果:p27在浸润性导管癌中的阳性表达率为36.0%(9/25),与乳腺导管上皮一般性增生的阳性表达率86.6%(13/15)、乳腺导管上皮不典型增生的阳性表达率82.1%(23/28)比较差异有统计学意义(P<0.05);β-catenin在浸润性导管癌中的阳性表达率为84.0%(21/25),与乳腺导管上皮一般性增生的阳性表达率20.0%(3/15)、乳腺导管上皮不典型增生的阳性表达率28.4%(8/28)比较差异有统计学意义(P<0.05);p21-ras在乳腺导管上皮一般性增生、乳腺导管上皮不典型增生、浸润性导管癌组织中的阳性表达率分别为13.3%(2/15)、32.1%(9/28)、68.0%(17/25),在浸润性导管癌中的阳性表达率与乳腺导管上皮一般性增生、乳腺导管上皮不典型增生的阳性表达率比较差异有统计学意义(P<0.05)。结论:p27、β-catenin、p21-ras基因表达异常与乳腺导管上皮癌变有关,可能是乳腺浸润性导管癌形成和发展的重要机制。

Objective： To investigate the expressions and their significance of p27, β-catenin, p21-ras in breast benign and maligant lesions. Methods： Immunohistochemical staining method of Envision was used to detect the p27, β-catenin and p21-ras gene expressions in 15 usual ductal hyperplasia, 28 atypical ductal hyperplasia and 25 breast cancer tissues. Results： Immunohistochemical staining method of Envision resuhs revealed that when compared with the p27 and β- catenin positive rates of breast cancer （36.0% and 84.0%, respectively） the p27 and β-catenin positive rates of atypical ductal hyperplasia （82. 1% and 28.5%, respective 32.1%（9/28）, were significantly lower （P 〈0.05）. The p21-ras positive rates was 13.3%（2/15）,32.1%（9/28）68. 0% （17/25） in usual ductal hyperplasia, atypical ductal hyperplasia and breast cancer respectively. The positive rates of p21-ras in breast benign and maligant lesions was significant （P〈 0.05）. Conclusion： It is suggested that the abnormal expressions of p27,β-catenin and p21-ras may play roles in the pathogenesis of breast carcinoma.