光果甘草异黄酮类成分光甘草定的制备工艺

Study on the Preparation Technology of Isoflavon Glabridin from Glycyrrhiza glabra L.

目的:研究提取和分离新疆光果甘草(Glycyrrhiza glabraL)的异黄酮类活性成分光甘草定(Glabrid-in,GB)的工艺。方法:用丙酮粗提光果甘草提取物,用氧化铝和硅胶柱层析法分离不同极性的组分,用制备型薄层层析法和重结晶法进行纯化,再用核磁共振、质谱、红外和紫外光谱法进行结构鉴定。结果:从光果甘草的干燥根中分离获得GB纯品,原制备工艺得到优化。结论:本方法在少量快速制备GB纯品中具有重要作用。

Objective： To study the extraction and separation method of active isoflavon constituent glabridin （GB） from Xinjiang Glycyrriza glabra root. Methods： We applied following methods as extraction by acetone, separation by aluminum oxide and silica gel chromatography, purification by preparative thin layer chromatography and by re-crystallization. The structural elucidation was performed by four spectrum methods （1H NMR, IR, UV, MS）. Results： Pure glabridin was obtained by above methods, and its chemical structure was determined by four spectrum methods;the preparation technology of GB in literature was optimized. Conclusion： Our study suggested that this procedure is one of the effective ways for obtaining high purity of glabridin.