摘要
用浸种、浸根、淋根、伤茎、伤叶和喷雾等接种方法测试枯草芽孢杆菌B-001菌株入侵烟草植株的途径,发现该菌可通过自然孔口和伤口入侵寄主植物;取接种植株的茎部组织切片在电镜下观察,发现该菌株在烟草的微管束和细胞内定殖。生长特性研究结果表明该菌株最适生长pH为7.5,最适生长温度为30℃,48～96h内处于稳定生长期。
The different inoculation methods,such as dipping seed,dipping root,watering root,injecting stem,injecting leaf,praying were used to test how the endophyte B-001（Bacillus subtilis） entered the host plant.The results indicated that the bacteria penetrated into plant tissues through natural wounds.Observing the plant tissue after inoculation 30 days by electron microscope,strain B001 could be found in vascular and cell.Study on its biological character showed that the optimal pH and temperature for the growth of B-001 were 7.5 and 30℃,respectively,and its stable growth stage was 48～96h.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第4期349-352,共4页
Journal of Nuclear Agricultural Sciences
基金
湖南省教育厅重点资助项目(02A019)
关键词
烟草
内生枯草芽孢杆菌B-001
内生定殖
生物学特性
tobacco
endophyte Bacillus subtilis B-001
endophytice colonization
biological characters
