无核雪柑与普通雪柑种胚发育过程蛋白质的差异

Specific proteins of "Wuhexuegan" and Citrus sinesis Osbeck at embryo development stage

采用考马斯亮蓝G-250法测定胚胎蛋白质含量，并用双向电泳技术分析胚胎的特异蛋白质。结果表明：无核雪柑种胚早期的蛋白质含量相对较高，其胚胎不同发育阶段出现了P1-P66个特异蛋白质；另外发现了4个特异于普通雪柑胚胎发育的蛋白质P11-P14。认为P11-P14与无核雪柑的无核密切相关，分别是P11（67.0 kD，pI5.1）、P12（65.5 kD，pI5.4）、P13（97.0 kD，pI5.8）及P14（64.3 kD，pI5.9）。

The method of Coomassie brilliant blue was used to determine the protein content in embryos. It indicated that the protein content in “Wuhexuegan” was higher than that in Citrus sinesis Osbeck at the early stage of embryo development. In order to study the specific proteins of “Wuhexuegan” at the embryonic development stage, two-di-mensional electrophoresis technique was employed. As a result, 6 new proteins at different stages of “Wuhexuegan” embryo development and 4 specific proteins of “Wuhexuegan” that were different from Citrus sinesis Osbeck in the embryonic development stage were identified. The 4 proteins were P11（67.0 kD, pI 5.1）, P12 （65.5kD, pI 5.4, P13 （97.0kD, pI 5.8）, and P14 （64.3kD, pI 5.9）. Therefore, it is suggested that these 4 specific proteins might play an important role in “Wuhexuegan” embryo differentiation and development.